Is there an easy way to do this, or am I doing something wrong?Īnother option is, I am planning to record this in a macro, since I do know the Total Wafer Area, I assume I could do some math to divide the Total void Area by the known wafer Area to get the Wafer % Voiding number I'm looking for outputted through the macro, but would need some help in how to do that.Īny help with this would be greatly appreciated. I would like it to tell me the % of Voiding in the wafer, which would be Total Void Area\ROI Area(wafer area). ![]() To quantify the amounts using unbiased stereology methods, you have to count the intersections of each of these subregions with regularly spaced grid points (black crosshairs in the image). ImageJ software, which is freeware Java based Image Processing software, has been used in this paper for particle size distribution analysis. using modified approach Image analysis is one of the best methods for measuring powder size distribution. But the %Area column # is low, I think because it's using the Total Area\the entire Image area. Thanks very helpful If I understand correctly, these are subregions of the tissue that you can’t distinguish in an automated fashion. 3 Flow diagram of particle dimensions measurement and plugin algorithm. I then run the Analyze Particles routine, it surrounds the voids well and in the summary table, the Total Area column seems to come out to the correct # for Total Void Area. ImageJ has comprehensive particle analysis algorithms which can be used effectively to count various biological particles. ![]() I then do a Threshold Color, to highlight only the voids. rectangle, circle, polygon or freeform) From the Analyze menu select set measurements. I have an image of a 4" diameter wafer with voiding. Note: ImageJ may be freely downloaded from here ( Select the cell of interest using any of the drawing/selection tools (i.e.
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